Gram Stain Report RequirementsThe following is an outline for the report. It will be graded out of a total of 100 points. Thereport should have appropriate font style and size (10-12), equal to or less than 1-inchmargins, appropriate indenting and at least three outside sources. Figures should have afigure number, title, reference. See below for specific information about each individual partof the report and the rubric which will be used for grading.Title Page(2.5 points)➢ should include the following information: Title of Report, Name of student, DatePurpose Statement➢ Why was the experiment conducted? (2.5points)o Name of the technique, what type of staining technique (simple or differential)?o What group of bacteria does it differentiate between? Based on what?Theory and background (55points)➢ Short history of the Gram stain. Who devised the technique, how? (5 of50 points)➢ What are the two major types of bacterial cell walls, what are their major and minor partsand their functions? Should include a figure of both types of cell walls.(20 of 50 points)➢ Describes what happens to the two types of cells (chemically speaking) at each step ofthe Gram stain. Should include a figure for better description.(20 of 50 points)o What happens to each cell type when crystal violet is applied?o What happens to each cell type as iodine is applied?o What happens to each cell type as decolorizer/alcohol is applied?o What happens to each cell type as safranin is applied?➢ What role does the Gram stain play in microbiology and medicine? (10 of50 points)Results(15 points)➢ This section should include 3 figures:o a) Gram stain of the Gram positive bacterial culture (S. aureus)o b) Gram stain of the Gram negative bacterial culture (E. coli)o c) Gram stain of the mixed bacterial culture (S. aureus and E. coli)➢ Figures can be hand drawn or from the web (appropriate references cited). Figures shouldhave a figure number, title, reference and should be labelled with arrows appropriately anddrawn in the right colors. Include the microscopic observations (color, shape andgrouping).➢ Summarize your results under every figure in a few sentences.Conclusions(15 points)➢ From your results, how can you categorize the microbes you view under the microscope?Which set of observations/characteristics belonged with each organism used?o What color do each of your bacterial samples appear as?o Are they Gram positive or Gram negative?o What are their shapes/arrangements?➢ How did you come to those conclusions? (tell me what lead you to that thought, connect itback to the principle of Gram Staining)o Why did each of your bacterial samples stain the particular color and whyReferences: (5points)➢ Correct reference format must be used (either APA or MLA).➢ References should be numbered, and the number added to the report when necessary.Spelling ofthe authors of the references must be correct.Formatting: (5points)➢ Overall format and quality of lab report, spelling and grammar➢ Use of proper scientific name formatting in the entire report including references.➢ Pages numbered, figures numbered, labelled and appropriately referenced in the varioussection of the report.GRADING RUBRIC:ITEM Points Worth(100)Titlepurpose statement2.52.5Theory and Background➢ History➢ Gram+ wall: structure description and figure➢ Gram- wall: structure description and figure➢ Principle of Staining of G+: description and figur➢ Principle of Staining of G- : description and figure➢ Role of Gram stain in microbiology and medicin55(5)(10)(10)(10)(10)(10)Results➢ Figures: 3 figures in correct colors with labels and description➢ Results summary15(9)(6Conclusions/Discussion➢ Results to concl.: for each of the 3 figures15(5)(5)➢ Which was which?: for each of the 3 figures match the organismto correct gram stain description➢ How do you know? for each of the 3 figures explainwhy G+ or G-(5)- proper in-house inclusion, number of outside references,formatting with URLs or pages included5Proper scientific nomenclature and formatting 2.5General formatting, spelling and grammar, pages numbered 2.5Total points 100
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